Date Received: Jun 16, 2025
Date Accepted: Sep 09, 2025
Date Published: Dec 31, 2025
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The Side-Effects of Polyvinylpyrrolidone-Coated Nanosilver Particles on Controlling Total Vibrio during Artemia parthenogenetica Hatching and Naupliar Development
,
Le Viet Dung (*)
2
Keywords
Artemia, development, nanosilver particles, toxicity, Vibrio
Abstract
Artemia parthenogenetica is an emerging species used in marine hatcheries in Vietnam. Disinfection during their cyst incubation is important, however, disinfectants might have side-effects. This study examined the effects of polyvinylpyrrolidone-coated nanosilver particles (AgNPs) on A. parthenogenetica cyst hatching and nauplii development. Cysts were exposed to low (0.1, 0.4, and 0.7 mg L-1) and high (1, 4, and 7 mg L-1) levels of AgNPs for 48h. The total Vibrio in the incubated water and nauplii significantly decreased when AgNPs were applied, however, the hatching success significantly reduced when the AgNPs level exceeded 1 mg L-1. Nauplii Instar I and Instar II were exposed to 10, 50, 100, 200, 500, 600, 700, 750, 800, 900, and 1000 mg L-1 to determine their LD50. The LD50-36h and LD50-48h of AgNPs on Instar I were 136.5 and 53.21 mg L-1, respectively, while those on Instar II were 22.15 and 12.1 mg L-1, respectively. Thus, the safe level of AgNPs, which could be used to control Vibrio during Artemia cyst incubation in fish/shrimp hatcheries, is 0.7-1 mg L-1.
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