This study evaluated the antibacterial and bactericidal activities of fresh aqueous extracts prepared from guava (Psidium guajava) leaves and fruits against two important freshwater fish pathogens, Edwardsiella ictaluri and Flavobacterium columnare. For each bacterial species, three strains were revived from the bacterial collection maintained at the Faculty of Fisheries, Vietnam National University of Agriculture, and were confirmed by colony morphology, biochemical characteristics, and species-specific polymerase chain reaction (PCR). Fresh extracts were obtained by mechanically pressing 100 g of guava leaves or fruits with 200 mL of distilled water, yielding 163 mL and 186 mL of extract, respectively. Antibacterial activity was assessed using the agar well diffusion method. The inhibition zones produced by the fruit extract against E. ictaluri and F. columnare measured 21.4 mm and 25.8 mm, respectively, and were significantly smaller than those produced by the leaf extract (26.7 mm and 30.8 mm, respectively). Both extracts exhibited strong antibacterial activity, comparable to that of oxytetracycline (30 µg). The minimum bactericidal concentrations (MBCs) of the fruit extract against E. ictaluri and F. columnare were 1:4 (12.5%) and 1:16 (3.13%), respectively. In contrast, the leaf extract showed bactericidal activity at lower concentrations, with MBCs of 1:8 (6.25%) for E. ictaluri and 1:32 (1.57%) for F. columnare. Overall, these findings demonstrate that fresh guava leaves extract, in particular, possesses strong antibacterial and bactericidal activities and has considerable potential for controlling bacterial diseases caused by E. ictaluri and F. columnare in freshwater aquaculture.