Selection of the promising homozygous glutinous thermo-sensitive genic sterile (waxy-TGMS) individuals from three segregating rice (Oryza sativa L.) populations

Date Received: Oct 21, 2025

Date Accepted: Mar 06, 2026

Date Published: Mar 31, 2026

DOI: https://doi.org/10.31817/vjas.2026.9.1.01

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Anh, N., Hong, N., Trung, N., Hoa, N. ., Lan, V., Xuan, T., … Quang, T. (2026). Selection of the promising homozygous glutinous thermo-sensitive genic sterile (waxy-TGMS) individuals from three segregating rice (Oryza sativa L.) populations. Vietnam Journal of Agricultural Sciences, 9(1), 2791–2803. https://doi.org/10.31817/vjas.2026.9.1.01

Selection of the promising homozygous glutinous thermo-sensitive genic sterile (waxy-TGMS) individuals from three segregating rice (Oryza sativa L.) populations

Nguyen Tuan Anh (*) 1 , Nguyen Thi Bich Hong 1 , Nguyen Quoc Trung 2 , Nguyen Thi Hoa 1 , Vu Ngoc Lan 1 , Tran Thi Thanh Xuan 1 , Vu Van Son 1 , Nguyen Van Hai 1 , Nguyen Thanh Tung 3   , Tran Van Quang 1

  • Corresponding author: [email protected]
  • 1 Faculty of Agronomy, Vietnam University of Agriculture, Hanoi 12400, Vietnam
  • 2 Faculty of Biotechnology, Vietnam University of Agriculture, Hanoi 12400, Vietnam
  • 3 Institute of Crop Development, Vietnam University of Agriculture, Hanoi 12400, Vietnam

    • Keywords

    TGMS, glutinous, two-line hybrid rice, marker-assisted selection

    Abstract


    The demand for glutinous rice for exporting encourages the breeding of new hybrid glutinous rice lines to meet the needed yield. Waxy-TGMS lines are principle materials to develop glutinous two-line hybrid rice, but have not been developed in Vietnam. This paper shows the selection of homozygous waxy-TGMS plants with advantageous agronomic traits from F2 segregating populations to develop promising waxy-TGMS breeding lines. More than 3000 individuals from three F2 populations derived from the crosses between non-waxy TGMS lines (tms5 gene donor) and glutinous rice varieties (wx gene donor) were screened to picked up only 10 homozygous waxy-TGMS plants (tms5/tms5 wx/wx) with desired agronomic characters. F3 seeds harvested from 10 desired F2 individuals all had low amylose content like their waxy male parent variety and could be used to develop further into ten waxy-TGMS breeding lines.

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