Received: Oct 21, 2025 / Accepted: Mar 06, 2026 / Published: Mar 31, 2026
The demand for glutinous rice for exporting encourages the breeding of new hybrid glutinous rice lines to meet the needed yield. Waxy-TGMS lines are principle materials to develop glutinous two-line hybrid rice, but have not been developed in Vietnam. This paper shows the selection of homozygous waxy-TGMS plants with advantageous agronomic traits from F2 segregating populations to develop promising waxy-TGMS breeding lines. More than 3000 individuals from three F2 populations derived from the crosses between non-waxy TGMS lines (tms5 gene donor) and glutinous rice varieties (wx gene donor) were screened to picked up only 10 homozygous waxy-TGMS plants (tms5/tms5 wx/wx) with desired agronomic characters. F3 seeds harvested from 10 desired F2 individuals all had low amylose content like their waxy male parent variety and could be used to develop further into ten waxy-TGMS breeding lines.