Promoter Analysis and Expression Patterns of the YABBY Transcription Factor Family in Cassava (Manihot esculenta) under Various Environmental Conditions

Date Received: Aug 02, 2024

Date Accepted: Jan 17, 2025

Date Published: Mar 31, 2025

DOI: https://doi.org/10.31817/vjas.2025.8.1.

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Issue: Vol. 8 No. 1 (2025): Vietnam Journal of Agricultural Sciences

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ENGINEERING AND TECHNOLOGY

How to Cite:

Hai, T., Trung, N., Bach, N., Gioi, D., Khanh, L., Khoa, T., … Diep, H. (2025). Promoter Analysis and Expression Patterns of the YABBY Transcription Factor Family in Cassava (Manihot esculenta) under Various Environmental Conditions. Vietnam Journal of Agricultural Sciences, 8(1), 2395–2403. https://doi.org/10.31817/vjas.2025.8.1.

Promoter Analysis and Expression Patterns of the YABBY Transcription Factor Family in Cassava (Manihot esculenta) under Various Environmental Conditions

Tong Van Hai 1 , Nguyen Quoc Trung 1 , Nguyen Duc Bach 1 , Dong Huy Gioi 1 , Le Duy Khanh 2 , Tran Dang Khoa 2 , Le Thanh Tinh 2 , Le Thi Ngoc Quynh 3 , Chu Duc Ha (*) 2   , Hoang Thi Diep 2

  • Corresponding author: cd.ha@vnu.edu.vn
  • 1 Faculty of Biotechnology, Vietnam National University of Agriculture, Hanoi 12400, Vietnam
  • 2 University of Engineering and Technology, Vietnam National University, Hanoi 11300, Vietnam
  • 3 Department of Biotechnology, Thuy Loi University, Hanoi 11500, Vietnam

    • Keywords

    Cassava, cis-regulatory element, expression level, transcription factor, YABBY

    Abstract


    The YABBY transcription factor (TF) family is a group of plant-specific proteins characterized by a unique structure comprised of a C2C2 zinc finger domain and a helix-loop-helix YABBY domain. These TFs play crucial roles in regulating various aspects of plant development, such as lateral organ formation and leaf polarity, as well as mediating responses to abiotic and biotic stresses. This study investigated the potential functions and expression profiles of 13 genes encoding the YABBY TF family in cassava (Manihot esculenta) under various stress conditions. Through a comprehensive analysis of promoter regions, we identified numerous cis-regulatory elements (CREs) associated with abiotic stress responses and phytohormone regulation. Specific CREs, such as low-temperature responsive elements and MYB recognize sites, were linked to cold and drought responses, respectively, suggesting their involvement in stress adaptation. RNA-Seq analysis under drought and PEG6000 treatments revealed significant transcriptional changes, with several YABBY genes being upregulated or downregulated, indicating their roles in drought tolerance and water use efficiency. Under biotic stress conditions, specifically cassava brown strike disease inoculation, YABBY genes exhibited diverse expression patterns, with notable downregulation of certain genes, suggesting their potential regulatory roles in stress responses. Taken together, these findings highlighted the diverse and critical functions of YABBY TFs in cassava's stress resilience and developmental processes.

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